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To isolate mutant animals, PCR amplification was performed using a single forward primer (5-TGAAAAGTCGATAAAAATTCAGTAGCA-3) and 2 reverse primers annealing specifically with the mutated (5-CTCAGTCGACGGTTATTTTGTA-3) or the WT sequence (5-ACCTTTTTACTGTACTTCAATTCGACT-3)
Some patients tolerate injections better on an empty stomach
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